Can Brf Cakes Touch Each Other

(Level 1 – beginners)

The PF-technique aka BRF-Tek (Brown Rice Flour technique) is a method developed by hobby mushroom cultivators for growing mushrooms in the simplest mode. Over the last decades many dissimilar versions of this method take adult. The following instruction is based on our experience.

Mushrooms demand a convenient substrate to develop fruit bodies. The substrate has to be produced under sterile weather to avoid contamination through mold or leaner. Contamination destroys non only the fungi but too the substrate. The substrate is inoculated with spores. In the next stage, the so chosen 'spawn run', the mycelium grows on the substrate. As soon as the substrate is fully colonised with mycelium, information technology is ready for the fruiting phase.

Recommended materials:

Microboxes or PF-jars
Measuring cup
Vermiculite
Brown rice flour
Sporesyringe (ATTENTION: not all mushroom species are suitable for cultivation on PF-substrate - pls. read product clarification!)
Fruiting chamber
Pressure cooker
Container for mixing
Water

Mixing the substrate

Blend one liter (nigh 700 k) of rice flour with 3 liters of Vermiculite medium, then add ane liter of water and mix again. That should result in 5 L of PF-substrate for 10 microboxes with 500 ml each.

Filling the substrate

Use your easily to fill the substrate into the boxes or glasses. Use your fingers to push the substrate into form to eliminate whatsoever hollow spaces, but don't printing too much. Fill the boxes or jars upwardly till i,5 cm under the border of the box remain free, and smoothen the surface of the substrate. Make clean the border of the box or jar with a wet piece of textile and fill the remainder with dry Vermiculite. The dry out Vermiculite acts as a filter to prevent intrusion of contamination from the outside into the substrate. Shut the boxes/jars. If you are using microboxes, call back to brand sure the lid is lying loosely on the box to ensure force per unit area balancing. If yous are using jars pierce the chapeau four times, i. e. make holes of about ii-3mm/0,1 inch in diameter, these volition later be used to inoculate the substrate.

Sterilise the substrate

A pressure cooker is used for the sterilising process. Overlying a 2-3 centimetre water level at the bottom of the cooker with a separating insert keeps the boxes/jars above the waterline. Assure yourself that at that place is no contact between the water and the boxes/jars, decant some water if necessary (listen the manufacturer information of your pressure level cooker!). Now fill in the jars or boxes. If the cooker is big enough you can brand two levels. Place the upper layer on a separating tray over the left spare zones of the lower layer. This ensures that the steam gets evenly distributed.

Become the lid locked and put the cooker on a hotplate and start the heating. Look until you can see steam leaking out, and so close the outlet. Start counting the sterilisation time from the moment the force per unit area gauge reaches its highest stage (for household pressure cookers). If yous have a professional person force per unit area steriliser at your disposal sterilise at 121°C/ 250°F/ xv psi/ ane.05 bar.

One time the sterilisation fourth dimension has passed the cooker must cool down in a clean place, preferably in front of a HEPA–filter (sterile air menses). If working without laminar flow hood, put a clean piece of cloth/newspaper, soaked with lxx % alcohol or a ten % chlorine solution,  over the cooker outlet during the cooling process to filter the air streaming into the pot while pressure level equalisation.

Afterwards the steriliser is fully depressurised, open the lid. The jars/boxes should cool down to room temperature in front of a HEPA-filter or in a Glove Box. Equally soon as the substrate has cooled down below 30°C/86°F it is prepare for inoculation. If you intend to continue later, close the boxes/jars tightly, store the substrates in the neatly make clean refrigerator (35.half-dozen-39.two°F, 2-iv°C) and use them within 4 weeks.

If y'all are using set sterilised PF-substrates, start here:

Inoculation of the substrate

Perform this piece of work step as hygienic as possible to prevent the substrate from being contaminatied by bacteria or molds. Make clean the worktop, launder your hands and forearms, put on face mask, hairnet and gloves and disinfect your worktop well.

Give the spore syringe a good shake to ensure that the spores are well suspended in the water. If using microboxes, you tin pierce the lid with the cannula (delight disinfect the spot you cull to pierce). The substrate jars will be inoculated through the 4 holes in the lid. If you use ready sterilised PF-substrates, please remove the tape from the holes in the lid before inoculating the substrate and leave the holes open afterwards, to ensure sufficient air exchange!

Remove the protective cover from the cannula simply before inoculation. You must not touch the sterile needle. Stick the needle through the holes of your box or jar towards the wall and inoculate the substrate, spreading the liquid along the wall of the box. Employ 1-4 ml of the spore-water-interruption per each box/jar (filled with 500 ml of substrate), portioned in all 4 holes.

Should the needle bear upon anything unsterile, you will accept to sterilise information technology over again. Therefore agree the nip of the needle into a flame (Bunsen burner or lighter) until the nip turns ruby-red and let it cool down once more. (Do not overheat the needle equally the stand is made of plastic which could melt!)

Mycelium growth

Afterward all substrates take been inoculated, they should be stored in a warm (upwards to 30°C) and dark place. The substrate is gear up for the adjacent step as soon as the whole box/jar is grown through with white mycelium (this unremarkably takes 2-iii weeks). Only the i,five cm layer of dry Vermiculite stays free of mycelium.

Fruiting

Rub off the layer of dry Vermiculite from the superlative of the boxes/jars and take out the mycelium. Put the mushroom mycelia into a growing sleeping accommodation or similar nether the convenient environmental conditions for the "primordia germination" stage. As presently every bit the first small mushrooms (primordia) have appeared, alter the temperature and humidity to the recommended fruiting weather condition. According to the species, it takes nigh 1 or more than weeks until the primordia announced, a few days afterwards you tin harvest your first ain mushrooms.

Source: http://mrca-science.org/index.php/en/mushroom-cultivation/43-zuchtanleitungen/90-pf-technique

Posted by: thainorome1942.blogspot.com

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